Journal: Neural Regeneration Research
Article Title: Neuroprotective effects of insulin-like growth factor-2 in 6-hydroxydopamine-induced cellular and mouse models of Parkinson’s disease
doi: 10.4103/1673-5374.355815
Figure Lengend Snippet: Changes in IGF-2 and IGF-2R expression in PD models. (A, B) In PD model mice, IGF-2 protein expression was reduced. (C, D) In SH-SY5Y cells, IGF-2 protein expression in the 6-OHDA group was reduced. (E, H) IGF-2R protein expression was also decreased in PD model mice. (F, I) Time-dependent immunoblotting study of the effect of 6-OHDA on IGF-2R expression in SH-SY5Y cells. (G, J) Effect of IGF-2 pretreatment on IGF-2R expression in SH-SY5Y cells subjected to 6-OHDA-induced injury. Western blot results showing the effect of different durations of IGF-2 pretreatment on IGF-2R expression 24 hours after 6-OHDA injury. All data are presented as mean ± SEM ( n ≥ 3).* P < 0.05, ** P < 0.01 (one-way analysis of variance followed by Tukey’s post hoc test). 6-OHDA: 6-Hydroxydopamine; IGF-2: insulin-like growth factor-2; IGF-2R: insulin-like growth factor-2 receptor; PD: Parkinson’s disease.
Article Snippet: The following primary antibodies were used: β-actin (mouse, 1:1000, Cat# ab8226, RRID: AB_306371, Abcam, Cambridge Science Park, Cambridge, UK), IGF-2 (rabbit, 1:1000, Cat# A2086, RRID: AB_2764106, ABclonal), IGF-2R (rabbit, 1:1000, Cat# A13022, RRID:AB_2759869, ABclonal), TH (rabbit, 1:5000, Cat# 25859-1-AP, RRID: AB_2716568, Proteintech), α-syn (rabbit, 1:1000, Cat# ab51253, RRID: AB_869973, Abcam), p-PI3K (rabbit, 1:1000, Cat# ab182651, RRID: AB_2756407, Abcam), p-AKT (rabbit, 1:1000, Cat# ab38449, RRID: AB_722678, Abcam), total PI3K (rabbit, 1:1000, Cat# ab182651, RRID: AB_2756407, Abcam), and total AKT (rabbit, 1:1000, Cat# ab8850, RRID: AB_2112117, Abcam).
Techniques: Expressing, Western Blot